Chromatography of Milk Proteins on Anion-Exchange Cellulose
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چکیده
منابع مشابه
Anion-Exchange Chromatography of Phosphopeptides: Weak Anion Exchange versus Strong Anion Exchange and Anion-Exchange Chromatography versus Electrostatic Repulsion–Hydrophilic Interaction Chromatography
Most phosphoproteomics experiments rely on prefractionation of tryptic digests before online liquid chromatography-mass spectrometry. This study compares the potential and limitations of electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) and anion-exchange chromatography (AEX). At a pH higher than 5, phosphopeptides have two negative charges per residue and are well-retained...
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Column chromatography has evolved to provide a rapid and effective alternative to more laborious methods for preparing high-quality DNA, such as CsCl-gradient centrifugation. This unit describes the use of a column made of a unique anion-exchange resin that selectively binds nucleic acids, allowing rapid separation of DNA from contaminating RNA, proteins, carbohydrates, and metabolites. The pro...
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The serum proteins designated as y-globulins include all proteins of the slowest moving of the several major protein groups seen on serum electrophoresis conducted at alkaline pH (2). Among the y-globulins of normal serum are proteins of differing electrophoretic mobility (3,4), proteins with sedimentation coefficients of 6.6 S and 18 S in the ultracentrifuge (2, 3, 5, 6)) many individual antib...
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LEVEL: INTERMEDIATE C hromatography columns can become contaminated by a variety of protein and nonprotein species during a purification campaign. Consequences of column contamination include an increase in backpressure, loss of signal resolution, altered product yield, and medium discoloration. Common chromatographic contaminants include • residual proteins • nucleic acids • lipids • endotoxin...
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ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 1961
ISSN: 0022-0302
DOI: 10.3168/jds.s0022-0302(61)89791-9